In the Greater Mekong Subregion (GMS), a growing demand for biofuels could help support the agriculture sector and provide an alternative source of energy. However, if deployed unsustainably, biofuels development can be associated with numerous risks that have negative ramifications for human development. This paper reviews existing literature and integrates various themes to provide an overview of four main issues related to biofuels deployment in the GMS: the need for alternative energy, risks to food security, considerations for environmental management, and opportunities for rural development. This paper was prepared as a discussion piece for the GMS 2020 International Conference (20-21 February 2012 in Bangkok, Thailand).